› 2o7g

X-ray diffraction
2.7Å resolution

Crystal structure of the Pribnow Box recognition region of SigC from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb2o7g/pdb
PDB entry 2o7g coloured by chain, front view.
PDB entry 2o7g coloured by chain, side view.
PDB entry 2o7g coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structural and biophysical studies on two promoter recognition domains of the extra-cytoplasmic function sigma factor sigma(C) from Mycobacterium tuberculosis.
Thakur KG, Joshi AM, Gopal B
J Biol Chem 282 4711-4718 (2007)
PMID: 17145760

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161475 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ECF RNA polymerase sigma factor SigC Chains: A, B
Molecule details ›
Chains: A, B
Length: 112 amino acids
Theoretical weight: 12.23 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGH1 (Residues: 1-112; Coverage: 61%)
Gene names: MTCY49.08, Rv2069, sigC
Sequence domains: Sigma-70 region 2
Structure domains: Rna Polymerase Sigma Factor; Chain: A

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P61
Unit cell:
a: 85.173Å b: 85.173Å c: 79.554Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.248
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The sigma factors of Mycobacterium tuberculosis: regulation of the regulators.
Sachdeva et al. (2010)
3 more

2 mentions without citation

Structural biology of bacterial RNA polymerase.
Murakami KS. (2015)
1 more