› 2ofb

X-ray diffraction
1.16Å resolution

Crystal structure of AVR4 (R112L/C122S)-BNA complex

Released:
DOI: 10.2210/pdb2ofb/pdb
PDB entry 2ofb coloured by chain, front view.
PDB entry 2ofb coloured by chain, side view.
PDB entry 2ofb coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Critical importance of loop conformation to avidin-enhanced hydrolysis of an active biotin ester.
Hayouka R, Eisenberg-Domovich Y, Hytönen VP, Määttä JA, Nordlund HR, Kulomaa MS, Wilchek M, Bayer EA, Livnah O
Acta Crystallogr D Biol Crystallogr 64 302-8 (2008)
PMID: 18323625

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Avidin-related protein 4/5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 126 amino acids
Theoretical weight: 14.11 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P56734 (Residues: 25-150; Coverage: 100%)
Gene names: AVR4, AVR5
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

2 bound ligands:
Ligand BNI 2 x BNI
Ligand FMT 8 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P41212
Unit cell:
a: 77.904Å b: 77.904Å c: 110.326Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.187
Expression system: Escherichia coli

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Citations

2 citation in other articles

Detailed characterization of the solution kinetics and thermodynamics of biotin, biocytin and HABA binding to avidin and streptavidin.
Delgadillo et al. (2019)
1 more