› 2ot0

X-ray diffraction
2.05Å resolution

Fructose-1,6-bisphosphate aldolase from rabbit muscle in complex with a C-terminal peptide of Wiskott-Aldrich syndrome protein

Released:
DOI: 10.2210/pdb2ot0/pdb
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PDB entry 2ot0 coloured by chain, front view.
PDB entry 2ot0 coloured by chain, side view.
PDB entry 2ot0 coloured by chain, top view.
Source organisms:
Primary publication:
A hydrophobic pocket in the active site of glycolytic aldolase mediates interactions with Wiskott-Aldrich syndrome protein.
St-Jean M, Izard T, Sygusch J
J Biol Chem 282 14309-15 (2007)
PMID: 17329259

Function and Biology Details

Reaction catalysed: 
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133667 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fructose-bisphosphate aldolase A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 363 amino acids
Theoretical weight: 39.26 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00883 (Residues: 2-364; Coverage: 100%)
Gene name: ALDOA
Sequence domains: Fructose-bisphosphate aldolase class-I
Structure domains: Aldolase class I
Actin nucleation-promoting factor WAS Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 15 amino acids
Theoretical weight: 1.78 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P42768 (Residues: 488-502; Coverage: 3%)
Gene names: IMD2, WAS

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 84.084Å b: 56.705Å c: 156.188Å
α: 90° β: 97.78° γ: 90°
R-values:
R R work R free
0.152 0.152 0.2
Expression systems:
  • Escherichia coli
  • Not provided

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Citations

14 citation in other articles

Fructose-bisphosphate aldolase a is a potential metastasis-associated marker of lung squamous cell carcinoma and promotes lung cell tumorigenesis and migration.
Du et al. (2014)
13 more

3 mentions without citation

Aldolase is essential for energy production and bridging adhesin-actin cytoskeletal interactions during parasite invasion of host cells.
Starnes et al. (2009)
2 more

PDB-REDO

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