› 2p09

X-ray diffraction
1.65Å resolution

Structural Insights into the Evolution of a Non-Biological Protein

Released:
DOI: 10.2210/pdb2p09/pdb
PDB entry 2p09 coloured by chain, front view.
PDB entry 2p09 coloured by chain, side view.
PDB entry 2p09 coloured by chain, top view.
Source organism: unidentified
Primary publication:
Structural insights into the evolution of a non-biological protein: importance of surface residues in protein fold optimization.
Smith MD, Rosenow MA, Wang M, Allen JP, Szostak JW, Chaput JC
OpenAccess logo PLoS One 2 e467 (2007)
PMID: 17520026

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163137 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
a non-biological ATP binding protein with two mutations N32D and D65V Chain: A
Molecule details ›
Chain: A
Length: 81 amino acids
Theoretical weight: 9.71 KDa
Source organism: unidentified
Expression system: Escherichia coli BL21(DE3)
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand CL 1 x CL
Ligand ATP 1 x ATP
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3221
Unit cell:
a: 72.791Å b: 72.791Å c: 54.752Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.177 0.195
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

In Vitro Selection of Peptides and Proteins-Advantages of mRNA Display.
Newton et al. (2020)
1 more

4 mentions without citation

Transplanting supersites of HIV-1 vulnerability.
Zhou et al. (2014)
3 more