› 2p7o

X-ray diffraction
1.44Å resolution

Crystal structure of genomically encoded fosfomycin resistance protein, FosX, from Listeria monocytogenes (tetragonal form)

Released:
DOI: 10.2210/pdb2p7o/pdb
PDB entry 2p7o coloured by chain, front view.
PDB entry 2p7o coloured by chain, side view.
PDB entry 2p7o coloured by chain, top view.
Source organism: Listeria monocytogenes EGD-e
Primary publication:
Structure and mechanism of the genomically encoded fosfomycin resistance protein, FosX, from Listeria monocytogenes.
Fillgrove KL, Pakhomova S, Schaab MR, Newcomer ME, Armstrong RN
Biochemistry 46 8110-20 (2007)
PMID: 17567049

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186969 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fosfomycin resistance protein FosX Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 15.59 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8Y6I2 (Residues: 1-133; Coverage: 100%)
Gene names: fosX, lmo1702
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P43
Unit cell:
a: 68.579Å b: 68.579Å c: 56.835Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.126 0.124 0.167
Expression system: Escherichia coli

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Citations

16 review citations

Fosfomycin.
Falagas et al. (2016)
15 more

1 mention without citation

Characterization of the genomically encoded fosfomycin resistance enzyme from Mycobacterium abscessus.
Travis et al. (2019)