› 2pjd

X-ray diffraction
2.1Å resolution

Crystal structure of 16S rRNA methyltransferase RsmC

Released:
DOI: 10.2210/pdb2pjd/pdb
PDB entry 2pjd coloured by chain, front view.
PDB entry 2pjd coloured by chain, side view.
PDB entry 2pjd coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Functional specialization of domains tandemly duplicated within 16S rRNA methyltransferase RsmC.
Sunita S, Purta E, Durawa M, Tkaczuk KL, Swaathi J, Bujnicki JM, Sivaraman J
OpenAccess logo Nucleic Acids Res 35 4264-74 (2007)
PMID: 17576679

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanine(1207) in 16S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(1207) in 16S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153879 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase C Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 37.9 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P39406 (Residues: 1-343; Coverage: 100%)
Gene names: JW4333, b4371, rsmC, yjjT
Sequence domains:
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 123.939Å b: 51.503Å c: 73.33Å
α: 90° β: 121.52° γ: 90°
R-values:
R R work R free
0.256 0.204 0.256
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structure, dynamics, and function of RNA modification enzymes.
Ishitani et al. (2008)
1 more

4 mentions without citation

Functional specialization of domains tandemly duplicated within 16S rRNA methyltransferase RsmC.
Sunita et al. (2007)
3 more