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Crystal Structure of a m1A58 tRNA methyltransferase

Source organism: Thermus thermophilus HB27
Biochemical function: tRNA (adenine(58)-N1)-methyltransferase activity
Biological process: methylation
Cellular component: tRNA (m1A) methyltransferase complex

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EC 2.1.1.220: tRNA (adenine(58)-N(1))-methyltransferase

Reaction catalysed:
S-adenosyl-L-methionine + adenine(58) in tRNA = S-adenosyl-L-homocysteine + N(1)-methyladenine(58) in tRNA
Systematic name:
S-adenosyl-L-methionine:tRNA (adenine(58)-N(1))-methyltransferase
Alternative Name(s):
  • Gcd10p-Gcd14p
  • Rv2118cp
  • Trm61p-Trm6p
  • TrmI
  • tRNA (m(1)A(58)) Mtase
  • tRNA (m(1)A(58)) methyltransferase
  • tRNA m(1)A(58) methyltransferase

GO terms

Sequence families

Pfam Protein families (Pfam)
PF08704
Domain description: tRNA methyltransferase complex GCD14 subunit
Occurring in:
  1. tRNA (adenine(58)-N(1))-methyltransferase TrmI
The deposited structure of PDB entry 2pwy contains 2 copies of Pfam domain PF08704 (tRNA methyltransferase complex GCD14 subunit) in tRNA (adenine(58)-N(1))-methyltransferase TrmI. Showing 1 copy in chain A.

PF14801
Domain description: TrmI-like N-terminal
Occurring in:
  1. tRNA (adenine(58)-N(1))-methyltransferase TrmI

InterPro InterPro annotations
IPR014816
Domain description: tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14
Occurring in:
  1. tRNA (adenine(58)-N(1))-methyltransferase TrmI
IPR029063
Domain description: S-adenosyl-L-methionine-dependent methyltransferase superfamily
Occurring in:
  1. tRNA (adenine(58)-N(1))-methyltransferase TrmI

Structure domains

CATH CATH domains
3.40.50.150
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: Vaccinia Virus protein VP39
Occurring in:
  1. tRNA (adenine(58)-N(1))-methyltransferase TrmI
The deposited structure of PDB entry 2pwy contains 2 copies of CATH domain 3.40.50.150 (Rossmann fold) in tRNA (adenine(58)-N(1))-methyltransferase TrmI. Showing 1 copy in chain A.
3.10.330.20
Class: Alpha Beta
Architecture: Roll
Topology: Vcp-like ATPase; Chain A, domain 2
Homology: Vcp-like ATPase; Chain A, domain 2
Occurring in:
  1. tRNA (adenine(58)-N(1))-methyltransferase TrmI
The deposited structure of PDB entry 2pwy contains 2 copies of CATH domain 3.10.330.20 (Vcp-like ATPase; Chain A, domain 2) in tRNA (adenine(58)-N(1))-methyltransferase TrmI. Showing 1 copy in chain A.

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