› 2q3m

X-ray diffraction
1.9Å resolution

Ensemble refinement of the protein crystal structure of an Arabidopsis thaliana putative steroid sulphotransferase

Released:
DOI: 10.2210/pdb2q3m/pdb
PDB entry 2q3m coloured by chain, ensemble of 16 models, front view.
PDB entry 2q3m coloured by chain, ensemble of 16 models, side view.
PDB entry 2q3m coloured by chain, ensemble of 16 models, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Ensemble refinement of protein crystal structures: validation and application.
Levin EJ, Kondrashov DA, Wesenberg GE, Phillips GN
Structure 15 1040-52 (2007)
PMID: 17850744

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156649 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosolic sulfotransferase 12 Chain: A
Molecule details ›
Chain: A
Length: 326 amino acids
Theoretical weight: 37.18 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P52839 (Residues: 1-326; Coverage: 100%)
Gene names: At2g03760, F19B11.21, RAR047, SOT12, ST1
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand MLA 1 x MLA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 91.477Å b: 120.899Å c: 74.315Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.157 0.217
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Enzymes in jasmonate biosynthesis - structure, function, regulation.
Schaller et al. (2009)
18 more

2 mentions without citation

phenix.model_vs_data: a high-level tool for the calculation of crystallographic model and data statistics.
Afonine et al. (2010)
1 more