› 2q57

X-ray diffraction
2Å resolution

X-ray structure of Cerulean GFP: A tryptophan-based chromophore useful for fluorescence lifetime imaging

Released:
DOI: 10.2210/pdb2q57/pdb
PDB entry 2q57 coloured by chain, front view.
PDB entry 2q57 coloured by chain, side view.
PDB entry 2q57 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
X-ray structure of Cerulean GFP: a tryptophan-based chromophore useful for fluorescence lifetime imaging.
Malo GD, Pouwels LJ, Wang M, Weichsel A, Montfort WR, Rizzo MA, Piston DW, Wachter RM
Biochemistry 46 9865-73 (2007)
PMID: 17685554

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 254 amino acids
Theoretical weight: 28.7 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRF 1 x CRF

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 51.232Å b: 63.339Å c: 69.821Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.188 0.262
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Newly engineered cyan fluorescent proteins with enhanced performances for live cell FRET imaging.
Mérola et al. (2014)
1 more

5 mentions without citation

Advances in imaging of understudied ions in signaling: A focus on magnesium.
Lazarou et al. (2020)
4 more