› 2q6t

X-ray diffraction
2.9Å resolution

Crystal structure of the Thermus aquaticus DnaB monomer

Released:
DOI: 10.2210/pdb2q6t/pdb
PDB entry 2q6t coloured by chain, front view.
PDB entry 2q6t coloured by chain, side view.
PDB entry 2q6t coloured by chain, top view.
Source organism: Thermus aquaticus
Primary publication:
The crystal structure of the Thermus aquaticus DnaB helicase monomer.
Bailey S, Eliason WK, Steitz TA
Nucleic Acids Res 35 4728-36 (2007)
PMID: 17606462

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195587 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Replicative DNA helicase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 444 amino acids
Theoretical weight: 48.8 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Z693 (Residues: 1-444; Coverage: 100%)
Gene names: BVI061214_00280, dnaC, dnab
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25, APS BEAMLINE 19-ID
Spacegroup: P3121
Unit cell:
a: 104.707Å b: 104.707Å c: 363.258Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.243 0.241 0.285
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

On helicases and other motor proteins.
Enemark et al. (2008)
4 more

7 mentions without citation

Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target.
Robinson et al. (2012)
6 more