2q8q

X-ray diffraction
2.15Å resolution

Crystal Structure of S. aureus IsdE complexed with heme

Released:
Primary publication:
Heme coordination by Staphylococcus aureus IsdE.
J Biol Chem 282 28815-28822 (2007)
PMID: 17666394

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-565487 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
High-affinity heme uptake system protein IsdE Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 29.69 KDa
Source organism: Staphylococcus aureus subsp. aureus N315
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7A652 (Residues: 32-289; Coverage: 95%)
Gene names: SA0980, isdE, sirF
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P43212
Unit cell:
a: 64.219Å b: 64.219Å c: 144.993Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.253
Expression system: Escherichia coli BL21