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High-resolution structure of the major periplasmic domain from the cell shape-determining filament MreC.

Lovering AL, Strynadka NCJ
J Mol Biol 372 1034-1044 (2007)
Cited: 14 times
EuropePMC logo PMID: 17707860

Abstract

Bacterial cell shape is dictated by the cell wall, a plastic structure that must adapt to growth and division whilst retaining its function as a selectively permeable barrier. The modulation of cell wall structure is achieved by a variety of enzymatic functions, all of which must be spatially regulated in a precise manner. The membrane-spanning essential protein MreC has been identified as the central hub in this process, linking the bacterial cytoskeleton to a variety of cell wall-modifying enzymes. Additionally, MreC can form filaments, believed to run perpendicularly to the membrane. We present here the 1.2 A resolution crystal structure of the major periplasmic domain of Streptococcus pneumoniae MreC. The protein shows a novel arrangement of two barrel-shaped domains, one of which shows homology to a known protein oligomerization motif, with the other resembling a catalytic domain from a bacterial protease. We discuss the implications of these results for MreC function, and detail the structural features of the molecule that may be responsible for the binding of partner proteins.

Reviews citing this publication (3)

  1. Structural perspective of peptidoglycan biosynthesis and assembly. Lovering AL, Safadi SS, Strynadka NC. Annu. Rev. Biochem. 81 451-478 (2012)
  2. Bridging cell wall biosynthesis and bacterial morphogenesis. Matteï PJ, Neves D, Dessen A. Curr. Opin. Struct. Biol. 20 749-755 (2010)
  3. Fortifying the wall: synthesis, regulation and degradation of bacterial peptidoglycan. Sobhanifar S, King DT, Strynadka NC. Curr. Opin. Struct. Biol. 23 695-703 (2013)

Articles citing this publication (11)

  1. The requirement for pneumococcal MreC and MreD is relieved by inactivation of the gene encoding PBP1a. Land AD, Winkler ME. J. Bacteriol. 193 4166-4179 (2011)
  2. Identification of dynamic structural motifs involved in peptidoglycan glycosyltransfer. Lovering AL, De Castro L, Strynadka NC. J. Mol. Biol. 383 167-177 (2008)
  3. Characterization of the elongasome core PBP2 : MreC complex of Helicobacter pylori. El Ghachi M, Matteï PJ, Ecobichon C, Martins A, Hoos S, Schmitt C, Colland F, Ebel C, Prévost MC, Gabel F, England P, Dessen A, Boneca IG. Mol. Microbiol. 82 68-86 (2011)
  4. Identification of EloR (Spr1851) as a regulator of cell elongation in Streptococcus pneumoniae. Stamsås GA, Straume D, Ruud Winther A, Kjos M, Frantzen CA, Håvarstein LS. Mol. Microbiol. 105 954-967 (2017)
  5. Enhanced cell disruption strategy in the release of recombinant hepatitis B surface antigen from Pichia pastoris using response surface methodology. Tam YJ, Allaudin ZN, Lila MA, Bahaman AR, Tan JS, Rezaei MA. BMC Biotechnol. 12 70 (2012)
  6. Molecular architecture of the PBP2-MreC core bacterial cell wall synthesis complex. Contreras-Martel C, Martins A, Ecobichon C, Trindade DM, Matteï PJ, Hicham S, Hardouin P, Ghachi ME, Boneca IG, Dessen A. Nat Commun 8 776 (2017)
  7. Self-association of MreC as a regulatory signal in bacterial cell wall elongation. Martins A, Contreras-Martel C, Janet-Maitre M, Miyachiro MM, Estrozi LF, Trindade DM, Malospirito CC, Rodrigues-Costa F, Imbert L, Job V, Schoehn G, Attrée I, Dessen A. Nat Commun 12 2987 (2021)
  8. Identification of potential regulatory domains within the MreC and MreD components of the cell elongation machinery. Rohs PDA, Qiu JM, Torres G, Smith MD, Fivenson EM, Bernhardt TG. J Bacteriol JB.00493-20 (2021)
  9. Allosteric activation of cell wall synthesis during bacterial growth. Shlosman I, Fivenson EM, Gilman MSA, Sisley TA, Walker S, Bernhardt TG, Kruse AC, Loparo JJ. Nat Commun 14 3439 (2023)
  10. Proteomic Investigation Uncovers Potential Targets and Target Sites of Pneumococcal Serine-Threonine Kinase StkP and Phosphatase PhpP. Hirschfeld C, Gómez-Mejia A, Bartel J, Hentschker C, Rohde M, Maaß S, Hammerschmidt S, Becher D. Front Microbiol 10 3101 (2019)
  11. The crystal structure of MreC provides insights into polymer formation. Xu Q, Sun N, Xiao Q, Huang CY, Xu M, Zhang W, Li L, Wang Q, Olieric V, Wang W, He J, Sun B. FEBS Open Bio 12 340-348 (2022)


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