› 2qnj

X-ray diffraction
2.7Å resolution

Kinase and Ubiquitin-associated domains of MARK3/Par-1

Released:
DOI: 10.2210/pdb2qnj/pdb
PDB entry 2qnj coloured by chain, front view.
PDB entry 2qnj coloured by chain, side view.
PDB entry 2qnj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Conformational instability of the MARK3 UBA domain compromises ubiquitin recognition and promotes interaction with the adjacent kinase domain.
Murphy JM, Korzhnev DM, Ceccarelli DF, Briant DJ, Zarrine-Afsar A, Sicheri F, Kay LE, Pawson T
Proc Natl Acad Sci U S A 104 14336-41 (2007)
PMID: 17726107

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151008 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MAP/microtubule affinity-regulating kinase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 328 amino acids
Theoretical weight: 37.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27448 (Residues: 44-44, 48-370; Coverage: 43%)
Gene names: CTAK1, EMK2, MARK3
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 62.675Å b: 112.544Å c: 117.206Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.215 0.279
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

The age of crosstalk: phosphorylation, ubiquitination, and beyond.
Hunter T. (2007)
9 more

12 mentions without citation

Domain Architecture of the Nonreceptor Tyrosine Kinase Ack1.
Kan et al. (2023)
11 more