› 2qno

X-ray diffraction
2Å resolution

Crystal Structure of the Mutant E55Q of the Cellulase CEL48F in Complex with a Thio-Oligosaccharide

Released:
DOI: 10.2210/pdb2qno/pdb
PDB entry 2qno coloured by chain, front view.
PDB entry 2qno coloured by chain, side view.
PDB entry 2qno coloured by chain, top view.
Source organism: Ruminiclostridium cellulolyticum
Primary publication:
Structures of mutants of cellulase Cel48F of Clostridium cellulolyticum in complex with long hemithiocellooligosaccharides give rise to a new view of the substrate pathway during processive action.
Parsiegla G, Reverbel C, Tardif C, Driguez H, Haser R
J Mol Biol 375 499-510 (2008)
PMID: 18035374

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-543863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endoglucanase F Chain: A
Molecule details ›
Chain: A
Length: 629 amino acids
Theoretical weight: 70.87 KDa
Source organism: Ruminiclostridium cellulolyticum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37698 (Residues: 30-658; Coverage: 91%)
Gene names: Ccel_0729, celCCF
Sequence domains: Glycosyl hydrolase family 48
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: SGC, BGC
Ligand SGC 5 x SGC
Ligand BGC 4 x BGC
1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 61.24Å b: 84.72Å c: 121.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.137 0.182
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Cellulosomes: highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates.
Fontes et al. (2010)
1 more

4 mentions without citation

Molecular and biochemical analyses of CbCel9A/Cel48A, a highly secreted multi-modular cellulase by Caldicellulosiruptor bescii during growth on crystalline cellulose.
Yi et al. (2013)
3 more