› 2qrs

X-ray diffraction
2Å resolution

Crystal Structure of a single chain trimer composed of the MHC I heavy chain H-2Kb Y84A, beta-2microglobulin, and ovalbumin-derived peptide.

Released:
DOI: 10.2210/pdb2qrs/pdb
PDB entry 2qrs coloured by chain, front view.
PDB entry 2qrs coloured by chain, side view.
PDB entry 2qrs coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural engineering of pMHC reagents for T cell vaccines and diagnostics.
Mitaksov V, Truscott SM, Lybarger L, Connolly JM, Hansen TH, Fremont DH
Chem Biol 14 909-22 (2007)
PMID: 17719490

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134970 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-2-microglobulin; H-2 class I histocompatibility antigen, K-B alpha chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 422 amino acids
Theoretical weight: 46.97 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01887 (Residues: 3-5, 21-119; Coverage: 85%)
  • Canonical: P01901 (Residues: 22-301; Coverage: 81%)
Gene names: B2m, H2-K, H2-K1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 66.1Å b: 89.3Å c: 88.5Å
α: 90° β: 111° γ: 90°
R-values:
R R work R free
0.21 0.21 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Basic and translational applications of engineered MHC class I proteins.
Hansen et al. (2010)
1 more

2 mentions without citation

Properties and applications of single-chain major histocompatibility complex class I molecules.
Kotsiou et al. (2011)
1 more