› 2qt2

X-ray diffraction
1.31Å resolution

Cyclized-Dehydrated Intermediate of GFP Variant Q183E in Chromophore Maturation

Released:
DOI: 10.2210/pdb2qt2/pdb
PDB entry 2qt2 coloured by chain, front view.
PDB entry 2qt2 coloured by chain, side view.
PDB entry 2qt2 coloured by chain, top view.
Source organism: Aequorea victoria
Entry authors: Wood TI, Barondeau DP, Hitomi C, Tainer JA, Getzoff ED

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.77 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand EDO 3 x EDO
1 modified residue:
Ligand CJO 1 x CJO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P212121
Unit cell:
a: 50.753Å b: 62.143Å c: 69.638Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.133 0.189
Expression system: Escherichia coli

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