› 2qwn

X-ray diffraction
2.4Å resolution

Crystal structure of disulfide-bond-crosslinked complex of bovine hsc70 (1-386aa)R171C and bovine Auxilin (810-910aa)D876C in the ADP*Pi state

Released:
DOI: 10.2210/pdb2qwn/pdb
PDB entry 2qwn coloured by chain, front view.
PDB entry 2qwn coloured by chain, side view.
PDB entry 2qwn coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structural basis of J cochaperone binding and regulation of Hsp70.
Jiang J, Maes EG, Taylor AB, Wang L, Hinck AP, Lafer EM, Sousa R
Mol Cell 28 422-33 (2007)
PMID: 17996706

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148505 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Heat shock cognate 71 kDa protein Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 43.37 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19120 (Residues: 1-394; Coverage: 61%)
Gene names: HSC70, HSPA8
Sequence domains: Hsp70 protein
Structure domains:
Auxilin Chain: B
Molecule details ›
Chain: B
Length: 94 amino acids
Theoretical weight: 10.94 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q27974 (Residues: 812-905; Coverage: 10%)
Gene name: DNAJC6
Structure domains: DnaJ domain

Ligands and Environments

4 bound ligands:
Ligand PO4 1 x PO4
Ligand NA 2 x NA
Ligand MG 1 x MG
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 38.627Å b: 58.262Å c: 225.929Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.208 0.279
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)

Quick links

Citations

36 review citations

The HSP70 chaperone machinery: J proteins as drivers of functional specificity.
Kampinga et al. (2010)
35 more

4 mentions without citation

Fine-tuning multiprotein complexes using small molecules.
Thompson et al. (2012)
3 more