› 2qxl

X-ray diffraction
2.41Å resolution

Crystal Structure Analysis of Sse1, a yeast Hsp110

Released:
DOI: 10.2210/pdb2qxl/pdb
PDB entry 2qxl coloured by chain, front view.
PDB entry 2qxl coloured by chain, side view.
PDB entry 2qxl coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Insights into Hsp70 chaperone activity from a crystal structure of the yeast Hsp110 Sse1.
Liu Q, Hendrickson WA
Cell 131 106-20 (2007)
PMID: 17923091

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152383 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat shock protein homolog SSE1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 658 amino acids
Theoretical weight: 73.57 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32589 (Residues: 2-659; Coverage: 95%)
Gene names: LPG3C, MSI3, SSE1, YPL106C
Sequence domains: Hsp70 protein
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand K 2 x K
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 218.045Å b: 125.331Å c: 74.708Å
α: 90° β: 98.67° γ: 90°
R-values:
R R work R free
0.197 0.195 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

28 review citations

Converging concepts of protein folding in vitro and in vivo.
Hartl et al. (2009)
27 more

27 mentions without citation

Structural frameworks for considering microbial protein- and nucleic acid-dependent motor ATPases.
Thomsen et al. (2008)
26 more