› 2qzx

X-ray diffraction
2.5Å resolution

Secreted aspartic proteinase (Sap) 5 from Candida albicans

Released:
DOI: 10.2210/pdb2qzx/pdb
PDB entry 2qzx coloured by chain, front view.
PDB entry 2qzx coloured by chain, side view.
PDB entry 2qzx coloured by chain, top view.
Source organisms:
Primary publication:
X-ray structures of Sap1 and Sap5: structural comparison of the secreted aspartic proteinases from Candida albicans.
Borelli C, Ruge E, Lee JH, Schaller M, Vogelsang A, Monod M, Korting HC, Huber R, Maskos K
Proteins 72 1308-19 (2008)
PMID: 18384081

Function and Biology Details

Reaction catalysed: 
Preferential cleavage at the carboxyl of hydrophobic amino acids, but fails to cleave 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17 and 24-Phe-|-Phe-25 of insulin B chain. Activates trypsinogen, and degrades keratin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-154944 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Secreted aspartic protease 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 342 amino acids
Theoretical weight: 37.24 KDa
Source organism: Candida albicans
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P43094 (Residues: 77-418; Coverage: 86%)
Gene names: CAALFM_C603030WA, CaO19.13032, CaO19.5585, SAP5
Sequence domains: Eukaryotic aspartyl protease
Structure domains: Acid Proteases
Pepstatin Chains: C, D
Molecule details ›
Chains: C, D
Length: 6 amino acids
Theoretical weight: 686 Da
Source organism: Streptomyces argenteolus subsp. toyonakensis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P41212
Unit cell:
a: 92.196Å b: 92.196Å c: 182.005Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.224 0.275
Expression systems:
  • Komagataella pastoris
  • Not provided

Quick links

Citations

3 review citations

Targeting virulence: a new paradigm for antifungals.
Gauwerky et al. (2009)
2 more

11 mentions without citation

Activity of Novel Synthetic Peptides against Candida albicans.
Lum et al. (2015)
10 more