› 2r09

X-ray diffraction
1.9Å resolution

Crystal Structure of Autoinhibited Form of Grp1 Arf GTPase Exchange Factor

Released:
DOI: 10.2210/pdb2r09/pdb
PDB entry 2r09 coloured by chain, front view.
PDB entry 2r09 coloured by chain, side view.
PDB entry 2r09 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis and mechanism of autoregulation in 3-phosphoinositide-dependent Grp1 family Arf GTPase exchange factors.
DiNitto JP, Delprato A, Gabe Lee MT, Cronin TC, Huang S, Guilherme A, Czech MP, Lambright DG
Mol Cell 28 569-83 (2007)
PMID: 18042453

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytohesin-3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 40.84 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O08967 (Residues: 63-399; Coverage: 85%)
Gene names: Cyth3, Grp1, Pscd3
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand SO4 7 x SO4
Ligand 4IP 2 x 4IP
Ligand PGE 1 x PGE
Ligand PE5 1 x PE5
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 83.599Å b: 94.611Å c: 115.308Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.241
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
24 more

10 mentions without citation

Translation of the phosphoinositide code by PI effectors.
Kutateladze TG. (2010)
9 more