› 2rce

X-ray diffraction
2.35Å resolution

DFP modified DegS delta PDZ

Released:
DOI: 10.2210/pdb2rce/pdb
PDB entry 2rce coloured by chain, front view.
PDB entry 2rce coloured by chain, side view.
PDB entry 2rce coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Sohn J, Grant RA, Sauer RT

Function and Biology Details

Reaction catalysed: 
Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142438 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine endoprotease DegS Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 243 amino acids
Theoretical weight: 26.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEE3 (Residues: 27-256; Coverage: 65%)
Gene names: JW3204, b3235, degS, hhoB, htrH
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MIS 9 x MIS

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 71.531Å b: 132.745Å c: 231.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.236
Expression system: Escherichia coli

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