› 2rtx

Solution NMR

Solution structure of the GGQ domain of YaeJ protein from Escherichia coli

Released:
DOI: 10.2210/pdb2rtx/pdb
PDB entry 2rtx coloured by chain, ensemble of 20 models, front view.
PDB entry 2rtx coloured by chain, ensemble of 20 models, side view.
PDB entry 2rtx coloured by chain, ensemble of 20 models, top view.
Source organism: Escherichia coli K-12
Primary publication:
Identification of residues required for stalled-ribosome rescue in the codon-independent release factor YaeJ.
Kogure H, Handa Y, Nagata M, Kanai N, Güntert P, Kubota K, Nameki N
OpenAccess logo Nucleic Acids Res 42 3152-63 (2014)
PMID: 24322300

Function and Biology Details

Reaction catalysed: 
N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-544559 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidyl-tRNA hydrolase ArfB Chain: A
Molecule details ›
Chain: A
Length: 121 amino acids
Theoretical weight: 13.47 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P40711 (Residues: 1-109; Coverage: 78%)
Gene names: JW0187, arfB, b0191, yaeJ
Sequence domains: RF-1 domain
Structure domains: Double Stranded RNA Binding Domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 92%
Refinement method: restrained energy minimization
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Mechanisms of ribosome rescue in bacteria.
Keiler KC. (2015)
11 more

2 mentions without citation

Carboxylic ester hydrolases: Classification and database derived from their primary, secondary, and tertiary structures.
Chen et al. (2016)
1 more