› 2snm

X-ray diffraction
1.97Å resolution

IN A STAPHYLOCOCCAL NUCLEASE MUTANT THE SIDE-CHAIN of A LYSINE REPLACING VALINE 66 IS FULLY BURIED IN THE HYDROPHOBIC CORE

Released:
DOI: 10.2210/pdb2snm/pdb
PDB entry 2snm coloured by chain, front view.
PDB entry 2snm coloured by chain, side view.
PDB entry 2snm coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
In a staphylococcal nuclease mutant the side-chain of a lysine replacing valine 66 is fully buried in the hydrophobic core.
Stites WE, Gittis AG, Lattman EE, Shortle D
J Mol Biol 221 7-14 (1991)
PMID: 1920420

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-523735 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.87 KDa
Source organism: Staphylococcus aureus
Expression system: Not provided
UniProt:
  • Canonical: P00644 (Residues: 83-231; Coverage: 73%)
Gene name: nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand THP 1 x THP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P41
Unit cell:
a: 47.156Å b: 47.156Å c: 62.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 not available not available
Expression system: Not provided

Quick links

Citations

12 review citations

What are the dielectric "constants" of proteins and how to validate electrostatic models?
Schutz et al. (2001)
11 more

9 mentions without citation

Development of constant-pH simulation methods in implicit solvent and applications in biomolecular systems.
Barroso daSilva et al. (2017)
8 more