› 2tio

X-ray diffraction
1.93Å resolution

LOW PACKING DENSITY FORM OF BOVINE BETA-TRYPSIN IN CYCLOHEXANE

Released:
DOI: 10.2210/pdb2tio/pdb
PDB entry 2tio coloured by chain, front view.
PDB entry 2tio coloured by chain, side view.
PDB entry 2tio coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
X-ray studies on two forms of bovine beta-trypsin crystals in neat cyclohexane.
Zhu G, Huang Q, Wang Z, Qian M, Jia Y, Tang Y
Biochim Biophys Acta 1429 142-50 (1998)
PMID: 9920392

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133319 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease 1 Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 23.32 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00760 (Residues: 24-246; Coverage: 97%)
Gene names: PRSS1, TRP1, TRY1, TRYP1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

4 bound ligands:
Ligand CA 1 x CA
Ligand SO4 3 x SO4
Ligand BEN 1 x BEN
Ligand HEX 1 x HEX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 63.02Å b: 63.78Å c: 69.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.179 0.228

Quick links

Citations

3 review citations

Improving enzymes by using them in organic solvents.
Klibanov AM. (2001)
2 more

4 mentions without citation

Fabrication of graphite via electrochemical conversion of CO2 in a CaCl2 based molten salt at a relatively low temperature.
Hu et al. (2019)
3 more