› 2uzj

X-ray diffraction
1.55Å resolution

Crystal structure of the mature streptococcal cysteine protease, mSpeB

Released:
DOI: 10.2210/pdb2uzj/pdb
PDB entry 2uzj coloured by chain, front view.
PDB entry 2uzj coloured by chain, side view.
PDB entry 2uzj coloured by chain, top view.
Source organism: Streptococcus pyogenes
Primary publication:
Structure of the mature Streptococcal cysteine protease exotoxin mSpeB in its active dimeric form.
Olsen JG, Dagil R, Niclasen LM, Sørensen OE, Kragelund BB
J Mol Biol 393 693-703 (2009)
PMID: 19712682

Function and Biology Details

Reaction catalysed: 
Preferential cleavage with hydrophobic residues at P2, P1 and P1'.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142885 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptopain Chains: A, B
Molecule details ›
Chains: A, B
Length: 253 amino acids
Theoretical weight: 27.61 KDa
Source organism: Streptococcus pyogenes
UniProt:
  • Canonical: P0C0J0 (Residues: 146-398; Coverage: 68%)
Gene name: speB
Sequence domains: Peptidase C10 family
Structure domains: Peptidase C10, streptopain

Ligands and Environments

1 bound ligand:
Ligand E64 2 x E64
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21
Unit cell:
a: 58.828Å b: 60.888Å c: 70.365Å
α: 90° β: 101.14° γ: 90°
R-values:
R R work R free
0.183 0.181 0.227

Quick links

Citations

3 review citations

Cysteine proteinase SpeB from Streptococcus pyogenes - a potent modifier of immunologically important host and bacterial proteins.
Nelson et al. (2011)
2 more

5 mentions without citation

Immunomodulating Enzymes from Streptococcus pyogenes-In Pathogenesis, as Biotechnological Tools, and as Biological Drugs.
Happonen et al. (2024)
4 more