› 2v19

X-ray diffraction
2.59Å resolution

Crystal structure of the T. thermophilus dodecin R45A mutant

Released:
DOI: 10.2210/pdb2v19/pdb
PDB entry 2v19 coloured by chain, front view.
PDB entry 2v19 coloured by chain, side view.
PDB entry 2v19 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
The dodecin from Thermus thermophilus, a bifunctional cofactor storage protein.
Meissner B, Schleicher E, Weber S, Essen LO
J Biol Chem 282 33142-54 (2007)
PMID: 17855371

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dodecin Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 68 amino acids
Theoretical weight: 7.54 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SIE3 (Residues: 2-69; Coverage: 99%)
Gene name: TTHA1431
Sequence domains: Dodecin
Structure domains: Flavin-binding protein dodecin

Ligands and Environments

3 bound ligands:
Ligand FMN 10 x FMN
Ligand COA 12 x COA
Ligand CL 5 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P212121
Unit cell:
a: 67.294Å b: 68.494Å c: 229.891Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.206 0.234
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

A survey of the year 2007 literature on applications of isothermal titration calorimetry.
Bjelić et al. (2008)
15 other articles