› 2v6u

X-ray diffraction
1.6Å resolution

High resolution crystal structure of pterin-4a-carbinolamine dehydratase from Toxoplasma gondii

Released:
DOI: 10.2210/pdb2v6u/pdb
PDB entry 2v6u coloured by chain, front view.
PDB entry 2v6u coloured by chain, side view.
PDB entry 2v6u coloured by chain, top view.
Source organism: Toxoplasma gondii RH
Primary publication:
Crystal structures of Toxoplasma gondii pterin-4a-carbinolamine dehydratase and comparisons with mammalian and parasite orthologues.
Cameron S, Fyffe SA, Goldie S, Hunter WN
Mol Biochem Parasitol 158 131-8 (2008)
PMID: 18215430

Function and Biology Details

Reaction catalysed: 
4a-hydroxytetrahydrobiopterin = 6,7-dihydrobiopterin + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173878 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4a-hydroxytetrahydrobiopterin dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 104 amino acids
Theoretical weight: 11.95 KDa
Source organism: Toxoplasma gondii RH
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2Q449 (Residues: 1-104; Coverage: 100%)
Gene names: TGRH88_045670, pcd
Sequence domains: Pterin 4 alpha carbinolamine dehydratase
Structure domains: Transcriptional coactivator/pterin dehydratase

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P43212
Unit cell:
a: 120.135Å b: 120.135Å c: 49.773Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.194
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Leishmania-macrophage interactions: insights into the redox biology.
Van Assche et al. (2011)
1 more