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Structural analysis of the Sulfolobus solfataricus MCM protein N-terminal domain.

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Liu W, Pucci B, Rossi M, Pisani FM, Ladenstein R
OpenAccess logo Nucleic Acids Res 36 3235-43 (2008)
Cited: 53 times
EuropePMC logo PMID: 18417534

Abstract

The Mini-Chromosome Maintenance (MCM) proteins are candidates of replicative DNA helicase in eukarya and archaea. Here we report a 2.8 A crystal structure of the N-terminal domain (residues 1-268) of the Sulfolobus solfataricus MCM (Sso MCM) protein. The structure reveals single-hexameric ring-like architecture, at variance from the protein of Methanothermobacter thermoautotrophicus (Mth). Moreover, the central channel in Sso MCM seems significantly narrower than the Mth counterpart, which appears to more favorably accommodate single-stranded DNA than double-stranded DNA, as supported by DNA-binding assays. Structural analysis also highlights the essential role played by the zinc-binding domain in the interaction with nucleic acids and allows us to speculate that the Sso MCM N-ter domain may function as a molecular clamp to grasp the single-stranded DNA passing through the central channel. On this basis possible DNA unwinding mechanisms are discussed.

Reviews - 2vl6 mentioned but not cited (4)

  1. The Mcm2-7 replicative helicase: a promising chemotherapeutic target. Simon NE, Schwacha A. Biomed Res Int 2014 549719 (2014)
  2. Biological Nanomotors with a Revolution, Linear, or Rotation Motion Mechanism. Guo P, Noji H, Yengo CM, Zhao Z, Grainge I. Microbiol Mol Biol Rev 80 161-186 (2016)
  3. Archaeal MCM Proteins as an Analog for the Eukaryotic Mcm2-7 Helicase to Reveal Essential Features of Structure and Function. Miller JM, Enemark EJ. Archaea 2015 305497 (2015)
  4. Unwinding 20 Years of the Archaeal Minichromosome Maintenance Helicase. Kelman LM, O'Dell WB, Kelman Z. J Bacteriol 202 e00729-19 (2020)

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Reviews citing this publication (11)

  1. The Mcm complex: unwinding the mechanism of a replicative helicase. Bochman ML, Schwacha A. Microbiol Mol Biol Rev 73 652-683 (2009)
  2. Mechanisms and regulation of DNA replication initiation in eukaryotes. Parker MW, Botchan MR, Berger JM. Crit Rev Biochem Mol Biol 52 107-144 (2017)
  3. Unwinding the structure and function of the archaeal MCM helicase. Sakakibara N, Kelman LM, Kelman Z. Mol Microbiol 72 286-296 (2009)
  4. The ring-shaped hexameric helicases that function at DNA replication forks. O'Donnell ME, Li H. Nat Struct Mol Biol 25 122-130 (2018)
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  6. Archaeal DNA replication. Kelman LM, Kelman Z. Annu Rev Genet 48 71-97 (2014)
  7. Insights into the MCM functional mechanism: lessons learned from the archaeal MCM complex. Brewster AS, Chen XS. Crit Rev Biochem Mol Biol 45 243-256 (2010)
  8. Structure and evolutionary origins of the CMG complex. Onesti S, MacNeill SA. Chromosoma 122 47-53 (2013)
  9. Origin DNA melting and unwinding in DNA replication. Gai D, Chang YP, Chen XS. Curr Opin Struct Biol 20 756-762 (2010)
  10. Structural Mechanisms of Hexameric Helicase Loading, Assembly, and Unwinding. Trakselis MA. F1000Res 5 F1000 Faculty Rev-111 (2016)
  11. DNA replication: Mechanisms and therapeutic interventions for diseases. Song HY, Shen R, Mahasin H, Guo YN, Wang DG. MedComm (2020) 4 e210 (2023)

Articles citing this publication (25)



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