› 2vsn

X-ray diffraction
2.75Å resolution

Structure and topological arrangement of an O-GlcNAc transferase homolog: insight into molecular control of intracellular glycosylation

Released:
DOI: 10.2210/pdb2vsn/pdb
PDB entry 2vsn coloured by chain, front view.
PDB entry 2vsn coloured by chain, side view.
PDB entry 2vsn coloured by chain, top view.
Source organism: Xanthomonas campestris pv. campestris str. 8004
Primary publication:
Structure of an O-GlcNAc transferase homolog provides insight into intracellular glycosylation.
Martinez-Fleites C, Macauley MS, He Y, Shen DL, Vocadlo DJ, Davies GJ
Nat Struct Mol Biol 15 764-5 (2008)
PMID: 18536723

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101641 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
protein O-GlcNAc transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 568 amino acids
Theoretical weight: 61.34 KDa
Source organism: Xanthomonas campestris pv. campestris str. 8004
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2XAK3 (Residues: 1-568; Coverage: 100%)
Gene name: XC_3364
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand UDP 2 x UDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX10.1
Spacegroup: P212121
Unit cell:
a: 86.691Å b: 105.692Å c: 151.036Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.241 0.265
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

22 review citations

Cross talk between O-GlcNAcylation and phosphorylation: roles in signaling, transcription, and chronic disease.
Hart et al. (2011)
21 more

5 mentions without citation

The hexosamine signaling pathway: O-GlcNAc cycling in feast or famine.
Hanover et al. (2010)
4 more