› 2vsq

X-ray diffraction
2.6Å resolution

Structure of surfactin A synthetase C (SrfA-C), a nonribosomal peptide synthetase termination module

Released:
DOI: 10.2210/pdb2vsq/pdb
PDB entry 2vsq coloured by chain, front view.
PDB entry 2vsq coloured by chain, side view.
PDB entry 2vsq coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of the termination module of a nonribosomal peptide synthetase.
Tanovic A, Samel SA, Essen LO, Marahiel MA
Science 321 659-63 (2008)
PMID: 18583577

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170691 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Surfactin synthase subunit 3 Chain: A
Molecule details ›
Chain: A
Length: 1304 amino acids
Theoretical weight: 147.44 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08787 (Residues: 1-1275; Coverage: 100%)
Gene names: BSU03510, srfA3, srfAC
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand LEU 1 x LEU
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2, SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 83.106Å b: 106.562Å c: 92.401Å
α: 90° β: 97.35° γ: 90°
R-values:
R R work R free
0.215 0.213 0.272
Expression system: Escherichia coli

Quick links

Citations

49 review citations

Natural functions of lipopeptides from Bacillus and Pseudomonas: more than surfactants and antibiotics.
Raaijmakers et al. (2010)
48 more

45 mentions without citation

Conformational dynamics in the Acyl-CoA synthetases, adenylation domains of non-ribosomal peptide synthetases, and firefly luciferase.
Gulick AM. (2009)
44 more