› 2vy1

X-ray diffraction
2.1Å resolution

Structure of LEAFY transcription factor from Arabidopsis thaliana in complex with DNA from AP1 promoter

Released:
DOI: 10.2210/pdb2vy1/pdb
PDB entry 2vy1 coloured by chain, front view.
PDB entry 2vy1 coloured by chain, side view.
PDB entry 2vy1 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis for LEAFY floral switch function and similarity with helix-turn-helix proteins.
Hamès C, Ptchelkine D, Grimm C, Thevenon E, Moyroud E, Gérard F, Martiel JL, Benlloch R, Parcy F, Müller CW
EMBO J 27 2628-37 (2008)
PMID: 18784751

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-507213 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Protein LEAFY Chain: A
Molecule details ›
Chain: A
Length: 194 amino acids
Theoretical weight: 21.89 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00958 (Residues: 227-420; Coverage: 46%)
Gene names: At5g61850, LFY, MAC9.18
Sequence domains: DNA Binding Domain (C-terminal) Leafy/Floricaula
Structure domains: Protein LEAFY
5'-D(*TP*TP*AP*CP*GP*GP*AP*CP*CP*AP *CP*TP*GP*GP*TP*CP*CP*TP*TP*CP)-3' Chain: W
Molecule details ›
Chain: W
Length: 20 nucleotides
Theoretical weight: 6.21 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P6522
Unit cell:
a: 98.39Å b: 98.39Å c: 176.44Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.224 0.252
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

The flowering of Arabidopsis flower development.
Irish VF. (2010)
3 more

6 mentions without citation

Plant transcription factors - being in the right place with the right company.
Strader et al. (2022)
5 more