2vy5 › Citations

Solution structure of the U11-48K CHHC zinc-finger domain that specifically binds the 5' splice site of U12-type introns.

Tidow H, Andreeva A, Rutherford TJ, Fersht AR
Structure 17 294-302 (2009)
Cited: 20 times
EuropePMC logo PMID: 19217400

Abstract

The formation of stable 18S U11/U12 di-snRNPs before their association with the pre-mRNA is a characteristic feature of the minor spliceosome. During the spliceosomal assembly, the 18S snRNP binds cooperatively to the introns' 5' splice and branch point site. The molecular basis for this recognition is still unknown. Here, we report the solution structure of the U11-48K CHHC Zn finger, a domain unique to the minor spliceosome. The CHHC Zn-finger structure revealed an unexpected similarity to the TFIIIA domains, with distinct features originating from the type and separation of the zinc-coordinating residues. We show that this domain specifically binds the 5' splice site sequence of U12-type introns when base paired to U11 snRNA in vitro and hence may contribute to the U12 intron recognition. We propose a model in which the U11-48K Zn finger stabilizes U11-5' splice site base pairing and thus plays an important role during the minor spliceosome assembly.

Articles - 2vy5 mentioned but not cited (2)

  1. Molecular architecture of human polycomb repressive complex 2. Ciferri C, Lander GC, Maiolica A, Herzog F, Aebersold R, Nogales E. Elife 1 e00005 (2012)
  2. The dipeptidyl peptidase IV inhibitors vildagliptin and K-579 inhibit a phospholipase C: a case of promiscuous scaffolds in proteins. Chakraborty S, Rendón-Ramírez A, Ásgeirsson B, Dutta M, Ghosh AS, Oda M, Venkatramani R, Rao BJ, Dandekar AM, Goñi FM. F1000Res 2 286 (2013)


Reviews citing this publication (6)

  1. The significant other: splicing by the minor spliceosome. Turunen JJ, Niemelä EH, Verma B, Frilander MJ. Wiley Interdiscip Rev RNA 4 61-76 (2013)
  2. The emerging role of minor intron splicing in neurological disorders. Jutzi D, Akinyi MV, Mechtersheimer J, Frilander MJ, Ruepp MD. Cell Stress 2 40-54 (2018)
  3. Conservation of the three-dimensional structure in non-homologous or unrelated proteins. Sousounis K, Haney CE, Cao J, Sunchu B, Tsonis PA. Hum Genomics 6 10 (2012)
  4. Ribonucleoprotein multimers and their functions. Bleichert F, Baserga SJ. Crit Rev Biochem Mol Biol 45 331-350 (2010)
  5. Developmental roles and molecular mechanisms of Asterix/GTSF1. Ipsaro JJ, Joshua-Tor L. Wiley Interdiscip Rev RNA 13 e1716 (2022)
  6. Investigations of Single-Subunit tRNA Methyltransferases from Yeast. Wang Z, Xu X, Li X, Fang J, Huang Z, Zhang M, Liu J, Qiu X. J Fungi (Basel) 9 1030 (2023)

Articles citing this publication (12)

  1. Minor class splicing shapes the zebrafish transcriptome during development. Markmiller S, Cloonan N, Lardelli RM, Doggett K, Keightley MC, Boglev Y, Trotter AJ, Ng AY, Wilkins SJ, Verkade H, Ober EA, Field HA, Grimmond SM, Lieschke GJ, Stainier DY, Heath JK. Proc Natl Acad Sci U S A 111 3062-3067 (2014)
  2. An ancient mechanism for splicing control: U11 snRNP as an activator of alternative splicing. Verbeeren J, Niemelä EH, Turunen JJ, Will CL, Ravantti JJ, Lührmann R, Frilander MJ. Mol Cell 37 821-833 (2010)
  3. The Arabidopsis U11/U12-65K is an indispensible component of minor spliceosome and plays a crucial role in U12 intron splicing and plant development. Jung HJ, Kang H. Plant J 78 799-810 (2014)
  4. The Arabidopsis homolog of human minor spliceosomal protein U11-48K plays a crucial role in U12 intron splicing and plant development. Xu T, Kim BM, Kwak KJ, Jung HJ, Kang H. J Exp Bot 67 3397-3406 (2016)
  5. GTSF-1 is required for formation of a functional RNA-dependent RNA Polymerase complex in Caenorhabditis elegans. Almeida MV, Dietz S, Redl S, Karaulanov E, Hildebrandt A, Renz C, Ulrich HD, König J, Butter F, Ketting RF. EMBO J 37 e99325 (2018)
  6. Mutations in the U11/U12-65K protein associated with isolated growth hormone deficiency lead to structural destabilization and impaired binding of U12 snRNA. Norppa AJ, Kauppala TM, Heikkinen HA, Verma B, Iwaï H, Frilander MJ. RNA 24 396-409 (2018)
  7. Asterix/Gtsf1 links tRNAs and piRNA silencing of retrotransposons. Ipsaro JJ, O'Brien PA, Bhattacharya S, Palmer AG, Joshua-Tor L. Cell Rep 34 108914 (2021)
  8. Alternative exon definition events control the choice between nuclear retention and cytoplasmic export of U11/U12-65K mRNA. Verbeeren J, Verma B, Niemelä EH, Yap K, Makeyev EV, Frilander MJ. PLoS Genet 13 e1006824 (2017)
  9. Isolation and expression of the human gametocyte-specific factor 1 gene (GTSF1) in fetal ovary, oocytes, and preimplantation embryos. Huntriss J, Lu J, Hemmings K, Bayne R, Anderson R, Rutherford A, Balen A, Elder K, Picton HM. J Assist Reprod Genet 34 23-31 (2017)
  10. A dual role of human tRNA methyltransferase hTrmt13 in regulating translation and transcription. Li H, Dong H, Xu B, Xiong QP, Li CT, Yang WQ, Li J, Huang ZX, Zeng QY, Wang ED, Liu RJ. EMBO J 41 e108544 (2022)
  11. Phylogenetic Analysis of the Plant U2 snRNP Auxiliary Factor Large Subunit A Gene Family in Response to Developmental Cues and Environmental Stimuli. Lu S, Gao C, Wang Y, He Y, Du J, Chen M, Zhao H, Fang H, Wang B, Cao Y. Front Plant Sci 12 739671 (2021)
  12. Phylogenetic analysis and stress response of the plant U2 small nuclear ribonucleoprotein B″ gene family. Gao C, Lu S, Zhou R, Ding J, Fan J, Han B, Chen M, Wang B, Cao Y. BMC Genomics 23 744 (2022)


Quick links