› 2w6k

X-ray diffraction
1.7Å resolution

The crystal structure at 1.7 A resolution of CobE, a protein from the cobalamin (vitamin B12) biosynthetic pathway

Released:
DOI: 10.2210/pdb2w6k/pdb
PDB entry 2w6k coloured by chain, front view.
PDB entry 2w6k coloured by chain, side view.
PDB entry 2w6k coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Entry authors: Vevodova J, Smith D, McGoldrick H, Deery E, Murzin AG, Warren MJ, Wilson KS

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191206 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CobE/GbiG C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 145 amino acids
Theoretical weight: 15.44 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HZQ0 (Residues: 1-142; Coverage: 100%)
Gene name: PA2947
Sequence domains: Cobalamin synthesis G C-terminus
Structure domains: CobE/GbiG C-terminal domain

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand GOL 3 x GOL
1 modified residue:
Ligand MSE 1 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1, ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 31.729Å b: 41.271Å c: 87.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.199 0.25
Expression system: Escherichia coli BL21(DE3)

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