› 2w95

X-ray diffraction
1.75Å resolution

STructure of the Discoidin I from Dictyostelium discoideum in complex with GalNAc at 1.75 angstrom resolution

Released:
DOI: 10.2210/pdb2w95/pdb
PDB entry 2w95 coloured by chain, front view.
PDB entry 2w95 coloured by chain, side view.
PDB entry 2w95 coloured by chain, top view.
Source organism: Dictyostelium discoideum AX2
Primary publication:
Discoidin I from Dictyostelium discoideum and Interactions with oligosaccharides: specificity, affinity, crystal structures, and comparison with discoidin II.
Mathieu SV, Aragão KS, Imberty A, Varrot A
J Mol Biol 400 540-54 (2010)
PMID: 20580724

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136334 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Discoidin-1 subunit A Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 254 amino acids
Theoretical weight: 28.35 KDa
Source organism: Dictyostelium discoideum AX2
Expression system: Escherichia coli
UniProt:
  • Canonical: P02886 (Residues: 1-253; Coverage: 100%)
Gene names: DDB_G0273063, DDB_G0273919, dscA-1, dscA-2
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand NGA 3 x NGA
Ligand MPD 4 x MPD
Ligand SO4 7 x SO4
Ligand CA 3 x CA
Ligand MRD 3 x MRD
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2221
Unit cell:
a: 72.404Å b: 274.047Å c: 86.136Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.193
Expression system: Escherichia coli

Quick links

Citations

8 review citations

X-linked juvenile retinoschisis: clinical diagnosis, genetic analysis, and molecular mechanisms.
Molday et al. (2012)
7 more

1 mention without citation

Discoidin I from Dictyostelium discoideum and Interactions with oligosaccharides: specificity, affinity, crystal structures, and comparison with discoidin II.
Mathieu et al. (2010)