› 2whj

X-ray diffraction
1.78Å resolution

Understanding how diverse mannanases recognise heterogeneous substrates

Released:
DOI: 10.2210/pdb2whj/pdb
PDB entry 2whj coloured by chain, front view.
PDB entry 2whj coloured by chain, side view.
PDB entry 2whj coloured by chain, top view.
Source organism: Salipaludibacillus agaradhaerens
Primary publication:
Understanding how diverse beta-mannanases recognize heterogeneous substrates.
Tailford LE, Ducros VM, Flint JE, Roberts SM, Morland C, Zechel DL, Smith N, Bjørnvad ME, Borchert TV, Wilson KS, Davies GJ, Gilbert HJ
Biochemistry 48 7009-18 (2009)
PMID: 19441796

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124184 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannan endo-1,4-beta-mannosidase Chain: A
Molecule details ›
Chain: A
Length: 308 amino acids
Theoretical weight: 34.24 KDa
Source organism: Salipaludibacillus agaradhaerens
Expression system: Bacillus subtilis
UniProt:
  • Canonical: G1K3N4 (Residues: 2-308; Coverage: 100%)
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand ACT 5 x ACT
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P61
Unit cell:
a: 111.97Å b: 111.97Å c: 47.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.122 0.119 0.173
Expression system: Bacillus subtilis

Quick links

Citations

9 review citations

The biochemistry and structural biology of plant cell wall deconstruction.
Gilbert HJ. (2010)
8 more

3 mentions without citation

Structural analysis of alkaline β-mannanase from alkaliphilic Bacillus sp. N16-5: implications for adaptation to alkaline conditions.
Zhao et al. (2011)
2 more