› 2whk

X-ray diffraction
1.7Å resolution

Structure of Bacillus subtilis mannanase man26

Released:
DOI: 10.2210/pdb2whk/pdb
PDB entry 2whk coloured by chain, front view.
PDB entry 2whk coloured by chain, side view.
PDB entry 2whk coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Understanding how diverse beta-mannanases recognize heterogeneous substrates.
Tailford LE, Ducros VM, Flint JE, Roberts SM, Morland C, Zechel DL, Smith N, Bjørnvad ME, Borchert TV, Wilson KS, Davies GJ, Gilbert HJ
Biochemistry 48 7009-18 (2009)
PMID: 19441796

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannan endo-1,4-beta-mannosidase Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 38.02 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O05512 (Residues: 27-362; Coverage: 100%)
Gene names: BSU05880, gmuG, ydhT
Sequence domains: Glycosyl hydrolase family 26
Structure domains: Glycosidases

Ligands and Environments

4 bound ligands:
Ligand GOL 2 x GOL
Ligand TRS 1 x TRS
Ligand CA 2 x CA
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P31
Unit cell:
a: 67.498Å b: 67.498Å c: 72.966Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.17 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

The biochemistry and structural biology of plant cell wall deconstruction.
Gilbert HJ. (2010)
8 more

3 mentions without citation

Structural and biochemical analyses of glycoside hydrolase families 5 and 26 β-(1,4)-mannanases from Podospora anserina reveal differences upon manno-oligosaccharide catalysis.
Couturier et al. (2013)
2 more