› 2wl9

X-ray diffraction
1.9Å resolution

Crystal structure of catechol 2,3-dioxygenase

Released:
DOI: 10.2210/pdb2wl9/pdb
PDB entry 2wl9 coloured by chain, front view.
PDB entry 2wl9 coloured by chain, side view.
PDB entry 2wl9 coloured by chain, top view.
Source organism: Rhodococcus sp. DK17
Primary publication:
Substrate binding mechanism of a type I extradiol dioxygenase.
Cho HJ, Kim K, Sohn SY, Cho HY, Kim KJ, Kim MH, Kim D, Kim E, Kang BS
J Biol Chem 285 34643-52 (2010)
PMID: 20810655

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180296 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
VOC domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 305 amino acids
Theoretical weight: 34.07 KDa
Source organism: Rhodococcus sp. DK17
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6REQ5 (Residues: 1-305; Coverage: 100%)
Gene name: akbC
Sequence domains:
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

4 bound ligands:
Ligand FE 4 x FE
Ligand MG 1 x MG
Ligand GOL 3 x GOL
Ligand MBD 2 x MBD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS
Spacegroup: P4
Unit cell:
a: 102.562Å b: 102.562Å c: 142.132Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.151 0.176
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 citation in other articles

A C⋅As lyase for degradation of environmental organoarsenical herbicides and animal husbandry growth promoters.
Yoshinaga et al. (2014)
10 more

1 mention without citation

Substrate binding mechanism of a type I extradiol dioxygenase.
Cho et al. (2010)