› 2wsn

X-ray diffraction
1.37Å resolution

Structure of Enhanced Cyan Fluorescent Protein at physiological pH

Released:
DOI: 10.2210/pdb2wsn/pdb
PDB entry 2wsn coloured by chain, front view.
PDB entry 2wsn coloured by chain, side view.
PDB entry 2wsn coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Intrinsic dynamics in ECFP and Cerulean control fluorescence quantum yield.
Lelimousin M, Noirclerc-Savoye M, Lazareno-Saez C, Paetzold B, Le Vot S, Chazal R, Macheboeuf P, Field MJ, Bourgeois D, Royant A
Biochemistry 48 10038-46 (2009)
PMID: 19754158

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.92 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRF 1 x CRF

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 51.182Å b: 62.72Å c: 70.593Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.142 0.14 0.185
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Photoswitching of E222Q GFP mutants: "concerted" mechanism of chromophore isomerization and protonation.
Abbruzzetti et al. (2010)
4 more

13 mentions without citation

ATP sensing in living plant cells reveals tissue gradients and stress dynamics of energy physiology.
De Col et al. (2017)
12 more