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Structural and functional characterization of human peripheral nervous system myelin protein P2.

<div class='caption-title'>SRCD analysis of human P2 conformation.</div>
<div class='caption-title'>Crystal structure of human P2.</div>
<div class='caption-title'>The solution structure of human P2 as analyzed by SAXS.</div>
Majava V, Polverini E, Mazzini A, Nanekar R, Knoll W, Peters J, Natali F, Baumgärtel P, Kursula I, Kursula P
OpenAccess logo PLoS One 5 e10300 (2010)
Cited: 35 times
EuropePMC logo PMID: 20421974

Abstract

The myelin sheath is a tightly packed multilayered membrane structure insulating selected axons in the central and the peripheral nervous systems. Myelin is a biochemically unique membrane, containing a specific set of proteins. In this study, we expressed and purified recombinant human myelin P2 protein and determined its crystal structure to a resolution of 1.85 A. A fatty acid molecule, modeled as palmitate based on the electron density, was bound inside the barrel-shaped protein. Solution studies using synchrotron radiation indicate that the crystal structure is similar to the structure of the protein in solution. Docking experiments using the high-resolution crystal structure identified cholesterol, one of the most abundant lipids in myelin, as a possible ligand for P2, a hypothesis that was proven by fluorescence spectroscopy. In addition, electrostatic potential surface calculations supported a structural role for P2 inside the myelin membrane. The potential membrane-binding properties of P2 and a peptide derived from its N terminus were studied. Our results provide an enhanced view into the structure and function of the P2 protein from human myelin, which is able to bind both monomeric lipids inside its cavity and membrane surfaces.

Articles - 2wut mentioned but not cited (6)

  1. Structural and functional characterization of human peripheral nervous system myelin protein P2. Majava V, Polverini E, Mazzini A, Nanekar R, Knoll W, Peters J, Natali F, Baumgärtel P, Kursula I, Kursula P. PLoS One 5 e10300 (2010)
  2. De novo PMP2 mutations in families with type 1 Charcot-Marie-Tooth disease. Motley WW, Palaima P, Yum SW, Gonzalez MA, Tao F, Wanschitz JV, Strickland AV, Löscher WN, De Vriendt E, Koppi S, Medne L, Janecke AR, Jordanova A, Zuchner S, Scherer SS. Brain 139 1649-1656 (2016)
  3. Atomic resolution view into the structure-function relationships of the human myelin peripheral membrane protein P2. Ruskamo S, Yadav RP, Sharma S, Lehtimäki M, Laulumaa S, Aggarwal S, Simons M, Bürck J, Ulrich AS, Juffer AH, Kursula I, Kursula P. Acta Crystallogr D Biol Crystallogr 70 165-176 (2014)
  4. Structural Basis for the Selective Inhibition of Cdc2-Like Kinases by CX-4945. Lee JY, Yun JS, Kim WK, Chun HS, Jin H, Cho S, Chang JH. Biomed Res Int 2019 6125068 (2019)
  5. Production and crystallization of a panel of structure-based mutants of the human myelin peripheral membrane protein P2. Lehtimäki M, Laulumaa S, Ruskamo S, Kursula P. Acta Crystallogr Sect F Struct Biol Cryst Commun 68 1359-1362 (2012)
  6. Cryo-EM, X-ray diffraction, and atomistic simulations reveal determinants for the formation of a supramolecular myelin-like proteolipid lattice. Ruskamo S, Krokengen OC, Kowal J, Nieminen T, Lehtimäki M, Raasakka A, Dandey VP, Vattulainen I, Stahlberg H, Kursula P. J Biol Chem 295 8692-8705 (2020)


Reviews citing this publication (5)

  1. The human fatty acid-binding protein family: evolutionary divergences and functions. Smathers RL, Petersen DR. Hum Genomics 5 170-191 (2011)
  2. Myelin Fat Facts: An Overview of Lipids and Fatty Acid Metabolism. Poitelon Y, Kopec AM, Belin S. Cells 9 E812 (2020)
  3. Evolution of genetic and genomic features unique to the human lineage. O'Bleness M, Searles VB, Varki A, Gagneux P, Sikela JM. Nat Rev Genet 13 853-866 (2012)
  4. Myelin-specific proteins: a structurally diverse group of membrane-interacting molecules. Han H, Myllykoski M, Ruskamo S, Wang C, Kursula P. Biofactors 39 233-241 (2013)
  5. Interaction of brain fatty acid-binding protein with the polyunsaturated fatty acid environment as a potential determinant of poor prognosis in malignant glioma. Elsherbiny ME, Emara M, Godbout R. Prog Lipid Res 52 562-570 (2013)

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  2. The Concise Guide to PHARMACOLOGY 2013/14: overview. Alexander SP, Benson HE, Faccenda E, Pawson AJ, Sharman JL, McGrath JC, Catterall WA, Spedding M, Peters JA, Harmar AJ, CGTP Collaborators, Abul-Hasn N, Anderson CM, Anderson CM, Araiksinen MS, Arita M, Arthofer E, Barker EL, Barratt C, Barnes NM, Bathgate R, Beart PM, Belelli D, Bennett AJ, Birdsall NJ, Boison D, Bonner TI, Brailsford L, Bröer S, Brown P, Calo G, Carter WG, Catterall WA, Chan SL, Chao MV, Chiang N, Christopoulos A, Chun JJ, Cidlowski J, Clapham DE, Cockcroft S, Connor MA, Cox HM, Cuthbert A, Dautzenberg FM, Davenport AP, Dawson PA, Dent G, Dijksterhuis JP, Dollery CT, Dolphin AC, Donowitz M, Dubocovich ML, Eiden L, Eidne K, Evans BA, Fabbro D, Fahlke C, Farndale R, Fitzgerald GA, Fong TM, Fowler CJ, Fry JR, Funk CD, Futerman AH, Ganapathy V, Gaisnier B, Gershengorn MA, Goldin A, Goldman ID, Gundlach AL, Hagenbuch B, Hales TG, Hammond JR, Hamon M, Hancox JC, Hauger RL, Hay DL, Hobbs AJ, Hollenberg MD, Holliday ND, Hoyer D, Hynes NA, Inui KI, Ishii S, Jacobson KA, Jarvis GE, Jarvis MF, Jensen R, Jones CE, Jones RL, Kaibuchi K, Kanai Y, Kennedy C, Kerr ID, Khan AA, Klienz MJ, Kukkonen JP, Lapoint JY, Leurs R, Lingueglia E, Lippiat J, Lolait SJ, Lummis SC, Lynch JW, MacEwan D, Maguire JJ, Marshall IL, May JM, McArdle CA, McGrath JC, Michel MC, Millar NS, Miller LJ, Mitolo V, Monk PN, Moore PK, Moorhouse AJ, Mouillac B, Murphy PM, Neubig RR, Neumaier J, Niesler B, Obaidat A, Offermanns S, Ohlstein E, Panaro MA, Parsons S, Pwrtwee RG, Petersen J, Pin JP, Poyner DR, Prigent S, Prossnitz ER, Pyne NJ, Pyne S, Quigley JG, Ramachandran R, Richelson EL, Roberts RE, Roskoski R, Ross RA, Roth M, Rudnick G, Ryan RM, Said SI, Schild L, Sanger GJ, Scholich K, Schousboe A, Schulte G, Schulz S, Serhan CN, Sexton PM, Sibley DR, Siegel JM, Singh G, Sitsapesan R, Smart TG, Smith DM, Soga T, Stahl A, Stewart G, Stoddart LA, Summers RJ, Thorens B, Thwaites DT, Toll L, Traynor JR, Usdin TB, Vandenberg RJ, Villalon C, Vore M, Waldman SA, Ward DT, Willars GB, Wonnacott SJ, Wright E, Ye RD, Yonezawa A, Zimmermann M. Br J Pharmacol 170 1449-1458 (2013)
  3. A role of peripheral myelin protein 2 in lipid homeostasis of myelinating Schwann cells. Zenker J, Stettner M, Ruskamo S, Domènech-Estévez E, Baloui H, Médard JJ, Verheijen MH, Brouwers JF, Kursula P, Kieseier BC, Chrast R. Glia 62 1502-1512 (2014)
  4. A Mutation in PMP2 Causes Dominant Demyelinating Charcot-Marie-Tooth Neuropathy. Hong YB, Joo J, Hyun YS, Kwak G, Choi YR, Yeo HK, Jwa DH, Kim EJ, Mo WM, Nam SH, Kim SM, Yoo JH, Koo H, Park HT, Chung KW, Choi BO. PLoS Genet 12 e1005829 (2016)
  5. Charge isomers of myelin basic protein: structure and interactions with membranes, nucleotide analogues, and calmodulin. Wang C, Neugebauer U, Bürck J, Myllykoski M, Baumgärtel P, Popp J, Kursula P. PLoS One 6 e19915 (2011)
  6. Enhanced axonal neuregulin-1 type-III signaling ameliorates neurophysiology and hypomyelination in a Charcot-Marie-Tooth type 1B mouse model. Scapin C, Ferri C, Pettinato E, Zambroni D, Bianchi F, Del Carro U, Belin S, Caruso D, Mitro N, Pellegatta M, Taveggia C, Schwab MH, Nave KA, Feltri ML, Wrabetz L, D'Antonio M. Hum Mol Genet 28 992-1006 (2019)
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  13. The Role of Peripheral Myelin Protein 2 in Remyelination. Stettner M, Zenker J, Klingler F, Szepanowski F, Hartung HP, Mausberg AK, Kleinschnitz C, Chrast R, Kieseier BC. Cell Mol Neurobiol 38 487-496 (2018)
  14. The myelin protein PMP2 is regulated by SOX10 and drives melanoma cell invasion. Graf SA, Heppt MV, Wessely A, Krebs S, Kammerbauer C, Hornig E, Strieder A, Blum H, Bosserhoff AK, Berking C. Pigment Cell Melanoma Res 32 424-434 (2019)
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  17. High-resolution structural model of porcine P2 myelin membrane protein with associated fatty acid ligand: fact or artifact? Sedzik J, Jastrzebski JP. J Neurosci Res 89 909-920 (2011)
  18. Identification of a pathogenic PMP2 variant in a multi-generational family with CMT type 1: Clinical gene panels versus genome-wide approaches to molecular diagnosis. Punetha J, Mackay-Loder L, Harel T, Coban-Akdemir Z, Jhangiani SN, Gibbs RA, Lee I, Terespolsky D, Lupski JR, Posey JE. Mol Genet Metab 125 302-304 (2018)
  19. Production, crystallization and neutron diffraction of fully deuterated human myelin peripheral membrane protein P2. Laulumaa S, Blakeley MP, Raasakka A, Moulin M, Härtlein M, Kursula P. Acta Crystallogr F Struct Biol Commun 71 1391-1395 (2015)
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