› 2x60

X-ray diffraction
2.8Å resolution

Crystal structure of T. maritima GDP-mannose pyrophosphorylase in complex with GTP.

Released:
DOI: 10.2210/pdb2x60/pdb
PDB entry 2x60 coloured by chain, front view.
PDB entry 2x60 coloured by chain, side view.
PDB entry 2x60 coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Structural insights into the catalytic mechanism of bacterial guanosine-diphospho-D-mannose pyrophosphorylase and its regulation by divalent ions.
Pelissier MC, Lesley SA, Kuhn P, Bourne Y
J Biol Chem 285 27468-27476 (2010)
PMID: 20573954

Function and Biology Details

Reaction catalysed: 
GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194691 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannose-1-phosphate guanylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 336 amino acids
Theoretical weight: 38.66 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9X0C3 (Residues: 1-336; Coverage: 100%)
Gene name: TM_1033
Sequence domains:
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

2 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 65.926Å b: 79.567Å c: 70.952Å
α: 90° β: 107.75° γ: 90°
R-values:
R R work R free
0.211 0.207 0.267
Expression system: Escherichia coli BL21

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Citations

2 review citations

GDP-Mannose Pyrophosphorylase: A Biologically Validated Target for Drug Development Against Leishmaniasis.
Pomel et al. (2019)
1 more

4 mentions without citation

The structural biology of enzymes involved in natural product glycosylation.
Singh et al. (2012)
3 more