› 2x6u

X-ray diffraction
1.9Å resolution

Crystal structure of human TBX5 in the DNA-free form

Released:
DOI: 10.2210/pdb2x6u/pdb
PDB entry 2x6u coloured by chain, front view.
PDB entry 2x6u coloured by chain, side view.
PDB entry 2x6u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of TBX5-DNA recognition: the T-box domain in its DNA-bound and -unbound form.
Stirnimann CU, Ptchelkine D, Grimm C, Müller CW
J Mol Biol 400 71-81 (2010)
PMID: 20450920

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189228 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
T-box transcription factor TBX5 Chain: A
Molecule details ›
Chain: A
Length: 203 amino acids
Theoretical weight: 23.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99593 (Residues: 50-251; Coverage: 39%)
Gene name: TBX5
Sequence domains: T-box
Structure domains: Transcription factor, T-box

Ligands and Environments

2 bound ligands:
Ligand PE4 1 x PE4
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 36.71Å b: 69.85Å c: 80.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.21 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

The T-box gene family: emerging roles in development, stem cells and cancer.
Papaioannou VE. (2014)
5 more

8 mentions without citation

Complex Interdependence Regulates Heterotypic Transcription Factor Distribution and Coordinates Cardiogenesis.
Luna-Zurita et al. (2016)
7 more