› 2xad

X-ray diffraction
1.7Å resolution

Crystal structure of deacetylase-teicoplanin complex in biosynthesis pathway of teicoplanin

Released:
DOI: 10.2210/pdb2xad/pdb
PDB entry 2xad coloured by chain, front view.
PDB entry 2xad coloured by chain, side view.
PDB entry 2xad coloured by chain, top view.
Source organisms:
Primary publication:
Regioselective deacetylation based on teicoplanin-complexed Orf2* crystal structures.
Chan HC, Huang YT, Lyu SY, Huang CJ, Li YS, Liu YC, Chou CC, Tsai MD, Li TL
Mol Biosyst 7 1224-31 (2011)
PMID: 21267472

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163311 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
LmbE family N-acetylglucosaminyl deacetylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 273 amino acids
Theoretical weight: 30.27 KDa
Source organism: Actinoplanes teichomyceticus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q6ZZJ1 (Residues: 1-273; Coverage: 100%)
Gene names: FHX34_108192, tcp14
Sequence domains: GlcNAc-PI de-N-acetylase
Structure domains: LmbE-like
TEICOPLANIN Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 7 amino acids
Theoretical weight: 1.21 KDa
Source organism: Nonomuraea gerenzanensis

Ligands and Environments

4 bound ligands:
Ligand BMA 4 x BMA
Ligand NAG 4 x NAG
Ligand GCS 4 x GCS
Ligand T55 4 x T55
4 modified residues:
Ligand MSE 16 x MSE
Ligand GHP 12 x GHP
Ligand 3MY 4 x 3MY
Ligand OMY 4 x OMY

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P1
Unit cell:
a: 58.775Å b: 70.713Å c: 76.313Å
α: 113.89° β: 108.29° γ: 98.2°
R-values:
R R work R free
0.203 0.201 0.237
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Opportunities for synthetic biology in antibiotics: expanding glycopeptide chemical diversity.
Thaker et al. (2015)
1 more

4 mentions without citation

Structure and function of the LmbE-like superfamily.
Viars et al. (2014)
3 more