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Rhamnogalacturonan lyase from Aspergillus aculeatus K150A active site mutant

Source organism: Aspergillus aculeatus
Biochemical function: rhamnogalacturonan endolyase activity
Biological process: cell wall organization
Cellular component: extracellular region

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EC 4.2.2.23: Rhamnogalacturonan endolyase

Reaction catalysed:
Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end.
Systematic name:
Alpha-L-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronate endolyase
Alternative Name(s):
  • Alpha-L-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronide lyase
  • RG-lyase
  • RGL4
  • Rgase B
  • Rgl11A
  • Rgl11Y
  • Rhamnogalacturonan alpha-L-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronide lyase
  • Rhamnogalacturonase B
  • RhiE
  • YesW

GO terms

Sequence families

Pfam Protein families (Pfam)
PF14683
Domain description: Polysaccharide lyase family 4, domain III
Occurring in:
  1. Rhamnogalacturonate lyase A
The deposited structure of PDB entry 2xhn contains 2 copies of Pfam domain PF14683 (Polysaccharide lyase family 4, domain III) in Rhamnogalacturonate lyase A. Showing 1 copy in chain A.

PF14686
Domain description: Polysaccharide lyase family 4, domain II
Occurring in:
  1. Rhamnogalacturonate lyase A
The deposited structure of PDB entry 2xhn contains 2 copies of Pfam domain PF14686 (Polysaccharide lyase family 4, domain II) in Rhamnogalacturonate lyase A. Showing 1 copy in chain A.

PF09284
Domain description: Rhamnogalacturonan lyase B, N-terminal
Occurring in:
  1. Rhamnogalacturonate lyase A
The deposited structure of PDB entry 2xhn contains 2 copies of Pfam domain PF09284 (Rhamnogalacturonan lyase B, N-terminal) in Rhamnogalacturonate lyase A. Showing 1 copy in chain A.

InterPro InterPro annotations
IPR016590
Domain description: Rhamnogalacturonase B
Occurring in:
  1. Rhamnogalacturonate lyase A
IPR029411
Domain description: Rhamnogalacturonan lyase, domain III
Occurring in:
  1. Rhamnogalacturonate lyase A
IPR008979
Domain description: Galactose-binding-like domain superfamily
Occurring in:
  1. Rhamnogalacturonate lyase A
IPR029413
Domain description: Rhamnogalacturonan lyase, domain II
Occurring in:
  1. Rhamnogalacturonate lyase A
IPR013784
Domain description: Carbohydrate-binding-like fold
Occurring in:
  1. Rhamnogalacturonate lyase A
IPR015364
Domain description: Rhamnogalacturonase B, N-terminal
Occurring in:
  1. Rhamnogalacturonate lyase A
IPR014718
Domain description: Glycoside hydrolase-type carbohydrate-binding
Occurring in:
  1. Rhamnogalacturonate lyase A
IPR011013
Domain description: Galactose mutarotase-like domain superfamily
Occurring in:
  1. Rhamnogalacturonate lyase A

Structure domains

CATH CATH domains
2.60.120.260
Class: Mainly Beta
Architecture: Sandwich
Topology: Jelly Rolls
Homology: Galactose-binding domain-like
Occurring in:
  1. Rhamnogalacturonate lyase A
The deposited structure of PDB entry 2xhn contains 2 copies of CATH domain 2.60.120.260 (Jelly Rolls) in Rhamnogalacturonate lyase A. Showing 1 copy in chain A.
2.60.40.1120
Class: Mainly Beta
Architecture: Sandwich
Topology: Immunoglobulin-like
Homology: Carboxypeptidase-like, regulatory domain
Occurring in:
  1. Rhamnogalacturonate lyase A
The deposited structure of PDB entry 2xhn contains 2 copies of CATH domain 2.60.40.1120 (Immunoglobulin-like) in Rhamnogalacturonate lyase A. Showing 1 copy in chain A.
2.70.98.10
Class: Mainly Beta
Architecture: Distorted Sandwich
Topology: Beta-galactosidase; Chain A, domain 5
Homology: Beta-galactosidase; Chain A, domain 5
Occurring in:
  1. Rhamnogalacturonate lyase A
The deposited structure of PDB entry 2xhn contains 2 copies of CATH domain 2.70.98.10 (Beta-galactosidase; Chain A, domain 5) in Rhamnogalacturonate lyase A. Showing 1 copy in chain A.

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