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Self-directed assembly and clustering of the cytoplasmic domains of inwardly rectifying Kir2.1 potassium channels on association with PSD-95.

Fomina S, Howard TD, Sleator OK, Golovanova M, O'Ryan L, Leyland ML, Grossmann JG, Collins RF, Prince SM
Biochim Biophys Acta 1808 2374-89 (2011)
Related entries: 1u4e, 1u4f, 2xkx

Cited: 8 times
EuropePMC logo PMID: 21756874

Abstract

The interaction of the extra-membranous domain of tetrameric inwardly rectifying Kir2.1 ion channels (Kir2.1NC(4)) with the membrane associated guanylate kinase protein PSD-95 has been studied using Transmission Electron Microscopy in negative stain. Three types of complexes were observed in electron micrographs corresponding to a 1:1 complex, a large self-enclosed tetrad complex and extended chains of linked channel domains. Using models derived from small angle X-ray scattering experiments in which high resolution structures from X-ray crystallographic and Nuclear Magnetic Resonance studies are positioned, the envelopes from single particle analysis can be resolved as a Kir2.1NC(4):PSD-95 complex and a tetrad of this unit (Kir2.1NC(4):PSD-95)(4). The tetrad complex shows the close association of the Kir2.1 cytoplasmic domains and the influence of PSD-95 mediated self-assembly on the clustering of these channels.

Articles citing this publication (8)

  1. Lengthening of the Stargazin Cytoplasmic Tail Increases Synaptic Transmission by Promoting Interaction to Deeper Domains of PSD-95. Hafner AS, Penn AC, Grillo-Bosch D, Retailleau N, Poujol C, Philippat A, Coussen F, Sainlos M, Opazo P, Choquet D. Neuron 86 475-489 (2015)
  2. Supertertiary structure of the synaptic MAGuK scaffold proteins is conserved. McCann JJ, Zheng L, Rohrbeck D, Felekyan S, Kühnemuth R, Sutton RB, Seidel CA, Bowen ME. Proc Natl Acad Sci U S A 109 15775-15780 (2012)
  3. Expression of Kir4.1 and Kir5.1 inwardly rectifying potassium channels in oligodendrocytes, the myelinating cells of the CNS. Brasko C, Hawkins V, De La Rocha IC, Butt AM. Brain Struct Funct 222 41-59 (2017)
  4. Protein crowding and lipid complexity influence the nanoscale dynamic organization of ion channels in cell membranes. Duncan AL, Reddy T, Koldsø H, Hélie J, Fowler PW, Chavent M, Sansom MSP. Sci Rep 7 16647 (2017)
  5. Synaptic MAGUK multimer formation is mediated by PDZ domains and promoted by ligand binding. Rademacher N, Kunde SA, Kalscheuer VM, Shoichet SA. Chem Biol 20 1044-1054 (2013)
  6. The Cryo-EM structure of the CorA channel from Methanocaldococcus jannaschii in low magnesium conditions. Cleverley RM, Kean J, Shintre CA, Baldock C, Derrick JP, Ford RC, Prince SM. Biochim Biophys Acta 1848 2206-2215 (2015)
  7. MAGUKs end a tale of promiscuity. Reissner C, Missler M. Proc Natl Acad Sci U S A 111 17350-17351 (2014)
  8. The Dual PDZ Domain from Postsynaptic Density Protein 95 Forms a Scaffold with Peptide Ligand. Rodzli NA, Lockhart-Cairns MP, Levy CW, Chipperfield J, Bird L, Baldock C, Prince SM. Biophys J 119 667-689 (2020)


Related citations provided by authors (1)

  1. Cytoplasmic domain structures of Kir2.1 and Kir3.1 show sites for modulating gating and rectification.. Pegan S, Arrabit C, Zhou W, Kwiatkowski W, Collins A, Slesinger PA, Choe S Nat Neurosci 8 279-87 (2005)

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