› 2xo8

X-ray diffraction
2.4Å resolution

Crystal Structure of Myosin-2 in Complex with Tribromodichloropseudilin

Released:
DOI: 10.2210/pdb2xo8/pdb
PDB entry 2xo8 coloured by chain, front view.
PDB entry 2xo8 coloured by chain, side view.
PDB entry 2xo8 coloured by chain, top view.
Source organism: Dictyostelium discoideum
Primary publication:
Inhibition of Myosin ATPase activity by halogenated pseudilins: a structure-activity study.
Preller M, Chinthalapudi K, Martin R, Knolker HJ, Manstein DJ
J Med Chem 54 3675-85 (2011)
PMID: 21534527

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myosin-2 heavy chain Chain: A
Molecule details ›
Chain: A
Length: 776 amino acids
Theoretical weight: 88.45 KDa
Source organism: Dictyostelium discoideum
Expression system: Dictyostelium discoideum
UniProt:
  • Canonical: P08799 (Residues: 3-761; Coverage: 36%)
Gene names: DDB_G0286355, mhcA
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand H70 1 x H70
Ligand AD9 1 x AD9
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: C2221
Unit cell:
a: 88.34Å b: 146.383Å c: 152.798Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.254
Expression system: Dictyostelium discoideum

Quick links

Citations

3 review citations

Nonmuscle myosin-2: mix and match.
Heissler et al. (2013)
2 more

5 mentions without citation

Mechanism and specificity of pentachloropseudilin-mediated inhibition of myosin motor activity.
Chinthalapudi et al. (2011)
4 more