› 2xpz

X-ray diffraction
2.3Å resolution

Structure of native yeast LTA4 hydrolase

Released:
DOI: 10.2210/pdb2xpz/pdb
PDB entry 2xpz coloured by chain, front view.
PDB entry 2xpz coloured by chain, side view.
PDB entry 2xpz coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
A leukotriene A4 hydrolase-related aminopeptidase from yeast undergoes induced fit upon inhibitor binding.
Helgstrand C, Hasan M, Uysal H, Haeggström JZ, Thunnissen MM
J Mol Biol 406 120-34 (2011)
PMID: 21146536

Function and Biology Details

Reaction catalysed: 
An epoxide + H(2)O = a glycol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-555703 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine aminopeptidase 2 Chain: A
Molecule details ›
Chain: A
Length: 632 amino acids
Theoretical weight: 72.9 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q10740 (Residues: 40-671; Coverage: 94%)
Gene names: LAP2, N2535, YNL045W
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand BU3 1 x BU3
Ligand ZN 1 x ZN
Ligand 15P 1 x 15P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P212121
Unit cell:
a: 70.833Å b: 98.096Å c: 99.197Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.269
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Crystal structures of the endoplasmic reticulum aminopeptidase-1 (ERAP1) reveal the molecular basis for N-terminal peptide trimming.
Kochan et al. (2011)
4 more

1 mention without citation

Marine Invertebrates: A Promissory Still Unexplored Source of Inhibitors of Biomedically Relevant Metallo Aminopeptidases Belonging to the M1 and M17 Families.
Pascual Alonso et al. (2023)