› 2y06

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE ANALYSIS OF THE ANTI-(4-HYDROXY-3-NITROPHENYL) - ACETYL MURINE GERMLINE ANTIBODY BBE6.12H3 FAB FRAGMENT IN COMPLEX WITH A PHAGE DISPLAY DERIVED DODECAPEPTIDE GDPRPSYISHLL

Released:
DOI: 10.2210/pdb2y06/pdb
PDB entry 2y06 coloured by chain, front view.
PDB entry 2y06 coloured by chain, side view.
PDB entry 2y06 coloured by chain, top view.
Source organisms:
Primary publication:
Structural elucidation of the mechanistic basis of degeneracy in the primary humoral response.
Khan T, Salunke DM
J Immunol 188 1819-27 (2012)
PMID: 22266283

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-211611 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ANTI-NP MURINE GERMLINE MONOCLONAL ANTIBODY BBE6.12H3, HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 220 amino acids
Theoretical weight: 23.46 KDa
Source organism: Mus musculus
ANTI-NP MURINE GERMLINE MONOCLONAL ANTIBODY BBE6.12H3, LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 211 amino acids
Theoretical weight: 22.54 KDa
Source organism: Mus musculus
PHAGE DISPLAY DERIVED ANTIGEN Chain: P
Molecule details ›
Chain: P
Length: 12 amino acids
Theoretical weight: 1.36 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2
Unit cell:
a: 55.57Å b: 61.88Å c: 112.5Å
α: 90° β: 90.55° γ: 90°
R-values:
R R work R free
0.234 0.234 0.266
Expression system: Not provided

Quick links

Citations

4 review citations

Specificity, polyspecificity, and heterospecificity of antibody-antigen recognition.
Van Regenmortel MH. (2014)
3 more

3 mentions without citation

Deprivation of the Periplasmic Chaperone SurA Reduces Virulence and Restores Antibiotic Susceptibility of Multidrug-Resistant Pseudomonas aeruginosa.
Klein et al. (2019)
2 more