› 2y3m

X-ray diffraction
2.3Å resolution

Structure of the extra-membranous domain of the secretin HofQ from Actinobacillus actinomycetemcomitans

Released:
DOI: 10.2210/pdb2y3m/pdb
PDB entry 2y3m coloured by chain, front view.
PDB entry 2y3m coloured by chain, side view.
PDB entry 2y3m coloured by chain, top view.
Source organism: Aggregatibacter actinomycetemcomitans
Primary publication:
The extra-membranous domains of the competence protein HofQ show DNA binding, flexibility and a shared fold with type I KH domains.
Tarry M, Jääskeläinen M, Paino A, Tuominen H, Ihalin R, Högbom M
J Mol Biol 409 642-53 (2011)
PMID: 21530539

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163350 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PROTEIN TRANSPORT PROTEIN HOFQ Chains: A, B
Molecule details ›
Chains: A, B
Length: 175 amino acids
Theoretical weight: 19.7 KDa
Source organism: Aggregatibacter actinomycetemcomitans
Expression system: Escherichia coli BL21
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P62
Unit cell:
a: 119.61Å b: 119.61Å c: 51.28Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.205 0.237
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Decoding the roles of pilotins and accessory proteins in secretin escort services.
Koo et al. (2012)
1 more

4 mentions without citation

Cryo-EM structure of the bifunctional secretin complex of Thermus thermophilus.
D'Imprima et al. (2017)
3 more