› 2y4v

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF HUMAN CALMODULIN IN COMPLEX WITH A DAP KINASE-1 MUTANT (W305Y) PEPTIDE

Released:
DOI: 10.2210/pdb2y4v/pdb
PDB entry 2y4v coloured by chain, front view.
PDB entry 2y4v coloured by chain, side view.
PDB entry 2y4v coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: de Diego I, Lehmann F, Wilmanns M

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143927 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-1 Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
Death-associated protein kinase 1 Chain: B
Molecule details ›
Chain: B
Length: 19 amino acids
Theoretical weight: 2.37 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P53355 (Residues: 302-320; Coverage: 1%)
Gene names: DAPK, DAPK1

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 65.561Å b: 33.923Å c: 74.257Å
α: 90° β: 92.21° γ: 90°
R-values:
R R work R free
0.23 0.229 0.238
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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