› 2y52

X-ray diffraction
1.65Å resolution

Crystal structure of E496A mutant of the box pathway encoded ALDH from Burkholderia xenovorans LB400

Released:
DOI: 10.2210/pdb2y52/pdb
PDB entry 2y52 coloured by chain, front view.
PDB entry 2y52 coloured by chain, side view.
PDB entry 2y52 coloured by chain, top view.
Source organism: Paraburkholderia xenovorans LB400
Primary publication:
Elucidating the reaction mechanism of the benzoate oxidation pathway encoded aldehyde dehydrogenase from Burkholderia xenovorans LB400.
Bains J, Leon R, Temke KG, Boulanger MJ
Protein Sci 20 1048-59 (2011)
PMID: 21495107

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171891 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde dehydrogenase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 534 amino acids
Theoretical weight: 55.78 KDa
Source organism: Paraburkholderia xenovorans LB400
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13WK4 (Residues: 1-531; Coverage: 100%)
Gene name: Bxe_A1420
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P1
Unit cell:
a: 58.05Å b: 68.13Å c: 78.08Å
α: 112.23° β: 90.56° γ: 111.69°
R-values:
R R work R free
0.183 0.18 0.231
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species.
Hettle et al. (2019)
2 more

1 mention without citation

Elucidating the reaction mechanism of the benzoate oxidation pathway encoded aldehyde dehydrogenase from Burkholderia xenovorans LB400.
Bains et al. (2011)